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rhPTH had a better effect on improving bone turnover level in DM mice. (A) Representative images of tartrate-resistant acid phosphatase (TRACP) staining of femur (n = 5 per group). Scale bar, 100 μm. (B) Percentage of TRACP positive area calculated by Image J program (n = 5 per group). (C, D) Serum procollagen type I intact N-terminal (P1NP) and C-terminal cross-linking telopeptide of type I collagen (CTX) concentrations determined by <t>ELISA</t> (n = 5 per group). CON, control mice; DM, type 2 diabetes mice; rhPTH, recombinant human parathyroid hormone; ALN, alendronate. Data are represented as means ± SD. * p <0.05, ** p <0.01, *** p <0.001.
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rhPTH had a better effect on improving bone turnover level in DM mice. (A) Representative images of tartrate-resistant acid phosphatase (TRACP) staining of femur (n = 5 per group). Scale bar, 100 μm. (B) Percentage of TRACP positive area calculated by Image J program (n = 5 per group). (C, D) Serum procollagen type I intact N-terminal (P1NP) and C-terminal cross-linking telopeptide of type I collagen (CTX) concentrations determined by <t>ELISA</t> (n = 5 per group). CON, control mice; DM, type 2 diabetes mice; rhPTH, recombinant human parathyroid hormone; ALN, alendronate. Data are represented as means ± SD. * p <0.05, ** p <0.01, *** p <0.001.
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rhPTH had a better effect on improving bone turnover level in DM mice. (A) Representative images of tartrate-resistant acid phosphatase (TRACP) staining of femur (n = 5 per group). Scale bar, 100 μm. (B) Percentage of TRACP positive area calculated by Image J program (n = 5 per group). (C, D) Serum procollagen type I intact N-terminal (P1NP) and C-terminal cross-linking telopeptide of type I collagen (CTX) concentrations determined by <t>ELISA</t> (n = 5 per group). CON, control mice; DM, type 2 diabetes mice; rhPTH, recombinant human parathyroid hormone; ALN, alendronate. Data are represented as means ± SD. * p <0.05, ** p <0.01, *** p <0.001.
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rhPTH had a better effect on improving bone turnover level in DM mice. (A) Representative images of tartrate-resistant acid phosphatase (TRACP) staining of femur (n = 5 per group). Scale bar, 100 μm. (B) Percentage of TRACP positive area calculated by Image J program (n = 5 per group). (C, D) Serum procollagen type I intact N-terminal (P1NP) and C-terminal cross-linking telopeptide of type I collagen (CTX) concentrations determined by ELISA (n = 5 per group). CON, control mice; DM, type 2 diabetes mice; rhPTH, recombinant human parathyroid hormone; ALN, alendronate. Data are represented as means ± SD. * p <0.05, ** p <0.01, *** p <0.001.

Journal: Frontiers in Endocrinology

Article Title: Effect of rhPTH(1-34) and alendronate on the treatment of type 2 diabetic bone disease

doi: 10.3389/fendo.2025.1657481

Figure Lengend Snippet: rhPTH had a better effect on improving bone turnover level in DM mice. (A) Representative images of tartrate-resistant acid phosphatase (TRACP) staining of femur (n = 5 per group). Scale bar, 100 μm. (B) Percentage of TRACP positive area calculated by Image J program (n = 5 per group). (C, D) Serum procollagen type I intact N-terminal (P1NP) and C-terminal cross-linking telopeptide of type I collagen (CTX) concentrations determined by ELISA (n = 5 per group). CON, control mice; DM, type 2 diabetes mice; rhPTH, recombinant human parathyroid hormone; ALN, alendronate. Data are represented as means ± SD. * p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: Serum insulin (nIU/mL) were detected using enzyme-linked immunosorbent assay (ELISA) kits from CUSABIO (Cusabio Biotech Cat# CSB-E05071m, RRID: AB_2916335, coefficient of variation of intra- and inter-assay <10%).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control, Recombinant